Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

What is the name of a man that was able to memorize all the phone books in England?

I remember learning about this man in my neuroscience courses when studying learning and cognition. From what I can remember, he is/was British, on the spectrum, worked for either the post...

21 April 2020 1,133 3 View

DdRAD library preparation- double bands after PCR?

09 March 2020 3,304 1 View

Would whole-proteome iTRAQ analysis be an appropriate method for validating and quantifying silencing activity of artificial miRNAs?

I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...

29 January 2020 9,994 3 View

How long can you store extracted xanthophyll samples?

Samples were extracted following Thayer and Bjorkmann 1990 using 80% cold acetone and were stored in a freezer in HPLC vials. Should I expect degradation of pigments with time?

11 November 2018 7,499 2 View

How can I collect blood from a neonatal mouse?

I would like to collect blood from a 1 day old mouse, terminal collection is fine because I also need to collect tissues. Can this be done by cardiac puncture or what method do people use to...

05 July 2018 4,326 16 View

Alternatives to ImageJ?

Are there more efficient techniques available, rather than ImageJ, for quantifying herbivory damage from photos of leaves? I have a basic understanding of machine learning but don't want to spend...

19 June 2018 3,021 3 View

Supplier for xanthophyll pigment standards?

I am trying to find a supplier in the US to purchase HPLC pigment standards for the xanthophyll cycle pigments, specifically antheraxanthin. I have found a supplier for zeaxanthin and...

31 October 2017 5,902 3 View

Has anyone used Dedoose qualitative analysis software? What did you think of it?

I'm a qualitative researcher not currently attached to a University and so without access to expensive, traditional QDA software. I'm looking for an affordable alternative that my employer won't...

12 October 2017 1,993 7 View

Is there a generally accepted substitution rate for fish mitochondrial DNA? Or, specifically, for ND2?

For divergence time estimation

15 March 2017 2,524 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View