Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

What is the name of a man that was able to memorize all the phone books in England?

I remember learning about this man in my neuroscience courses when studying learning and cognition. From what I can remember, he is/was British, on the spectrum, worked for either the post...

21 April 2020 1,133 3 View

DdRAD library preparation- double bands after PCR?

09 March 2020 3,304 1 View

Would whole-proteome iTRAQ analysis be an appropriate method for validating and quantifying silencing activity of artificial miRNAs?

I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...

29 January 2020 9,994 3 View

How long can you store extracted xanthophyll samples?

Samples were extracted following Thayer and Bjorkmann 1990 using 80% cold acetone and were stored in a freezer in HPLC vials. Should I expect degradation of pigments with time?

11 November 2018 7,499 2 View

How can I collect blood from a neonatal mouse?

I would like to collect blood from a 1 day old mouse, terminal collection is fine because I also need to collect tissues. Can this be done by cardiac puncture or what method do people use to...

05 July 2018 4,326 16 View

Alternatives to ImageJ?

Are there more efficient techniques available, rather than ImageJ, for quantifying herbivory damage from photos of leaves? I have a basic understanding of machine learning but don't want to spend...

19 June 2018 3,021 3 View

Supplier for xanthophyll pigment standards?

I am trying to find a supplier in the US to purchase HPLC pigment standards for the xanthophyll cycle pigments, specifically antheraxanthin. I have found a supplier for zeaxanthin and...

31 October 2017 5,902 3 View

Has anyone used Dedoose qualitative analysis software? What did you think of it?

I'm a qualitative researcher not currently attached to a University and so without access to expensive, traditional QDA software. I'm looking for an affordable alternative that my employer won't...

12 October 2017 1,993 7 View

Is there a generally accepted substitution rate for fish mitochondrial DNA? Or, specifically, for ND2?

For divergence time estimation

15 March 2017 2,524 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View