Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

What is the name of a man that was able to memorize all the phone books in England?

I remember learning about this man in my neuroscience courses when studying learning and cognition. From what I can remember, he is/was British, on the spectrum, worked for either the post...

21 April 2020 1,133 3 View

DdRAD library preparation- double bands after PCR?

09 March 2020 3,304 1 View

Would whole-proteome iTRAQ analysis be an appropriate method for validating and quantifying silencing activity of artificial miRNAs?

I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...

29 January 2020 9,994 3 View

How long can you store extracted xanthophyll samples?

Samples were extracted following Thayer and Bjorkmann 1990 using 80% cold acetone and were stored in a freezer in HPLC vials. Should I expect degradation of pigments with time?

11 November 2018 7,499 2 View

How can I collect blood from a neonatal mouse?

I would like to collect blood from a 1 day old mouse, terminal collection is fine because I also need to collect tissues. Can this be done by cardiac puncture or what method do people use to...

05 July 2018 4,326 16 View

Alternatives to ImageJ?

Are there more efficient techniques available, rather than ImageJ, for quantifying herbivory damage from photos of leaves? I have a basic understanding of machine learning but don't want to spend...

19 June 2018 3,021 3 View

Supplier for xanthophyll pigment standards?

I am trying to find a supplier in the US to purchase HPLC pigment standards for the xanthophyll cycle pigments, specifically antheraxanthin. I have found a supplier for zeaxanthin and...

31 October 2017 5,902 3 View

Has anyone used Dedoose qualitative analysis software? What did you think of it?

I'm a qualitative researcher not currently attached to a University and so without access to expensive, traditional QDA software. I'm looking for an affordable alternative that my employer won't...

12 October 2017 1,993 7 View

Can anyone give me a protocol for cell viability assay using propidium iodide by FACS?

I am testing whether a knockdown causes cell death and want to look at the cell viability using propidium iodide, which I can analyze by FACS. Does anyone have a detailed protocol for this?

19 April 2017 1,575 2 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View