The only time I ever saw this effect was on an agarose gel prepared using water instead of TAE/TBE

It also looks like you're loading too much in the well, 50 ng of plasmid should be enough to be seen with EtBr or SYBR Safe staining.

Hi Saleheen Khan ,

I agree with Ciaran Daly that the gel was overloaded. From your image alone, it's unclear if the plasmid was cut. You need to load a non digested control. Then you would see a shift to a higher molecular weight. You should not use more than 500 ng in the gel and that is already a lot.

kind regards

Soenke

Hi

If you are concerned about the jaggy appearance of the bands, it is normal when linearization happens.

I think you should isolate plasmid freshly and try it again. At first make sure HindIII site is unique site in your plasmid. Simply copy the sequences of plasmid and paste in NEB cutter