Should I use B/B0 to calculate ELISA results?

11 November 2017 1 5K Report

I did my experiment using a competitive enzime ELISA kit and I want to know if I have to calculate B/Bo to compare sample intensities to obtain a percentage bound. Or could I just use the results obtained from the curve?

Michael G. Weller

If you performed your calculations properly, you should obtain the same results for your samples.

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