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Questions related from Ana Dias
My experimental protocol is as follows: I have two groups: - CTL group (sleep and wake ad libitum), rats euthanized in six different ZTs --> ZT0, ZT4, ZT8, ZT12, ZT16 e ZT20. Sleep...
09 September 2018 1,409 6 View
Bom dia, Fiz o seguinte experimento com 2 diferentes grupos: Ratos controle (sono e vigília a vontade), eutanasiados em 6 diferentes ZTs --> ZT0, ZT4, ZT8, ZT12, ZT16 e ZT20. Ratos privados...
09 September 2018 8,755 1 View
I´m having some trouble with my Western Blot experiments (SDS page). I would like to know what are the steps to standardize a reaction. I tested one sample with beta actin antibody and it shows 2...
04 April 2018 146 3 View
I did my experiment using a competitive enzime ELISA kit and I want to know if I have to calculate B/Bo to compare sample intensities to obtain a percentage bound. Or could I just use the results...
11 November 2017 5,249 1 View
I`ve done an ELISA experiment (competitive enzime) and I want to know how to verify if everything is ok. For example, how to verify if positive control was adequate (1,785) Also, If the readings...
11 November 2017 3,060 4 View
I did an ELISA experiment using a kit for MCH in rat CSF. The kit recommends using 50 μL sample for each well but since I will also dose orexin, I had to decrease the amount of pipetted sample....
08 August 2017 9,784 3 View
I would like to know if the mean of my delta delta Ct for my control group (non treated) normalized to the endogenous control must always be equal to 1.
05 May 2014 6,498 17 View
