Any suggestions would be helpful.
Thanks and warmest regards.
Dear all, good day! I have a question regarding the use of NaOH for lysis of mammalian cells. Is it a common practice? Are there any publications which have used NaOH to perform cell lysis? If...
03 April 2019 5,332 4 View
Dear all, recently i did a scratch test migration assay on my test compounds incubated with my cell line of interest and it appeared that the cell line seem to exhibit signs of increased cell...
10 November 2018 382 5 View
Dear all, recently i am considering if i have been doing my MTT assay wrongly as i have been using my media (with 10% FBS) for my cell viability assay. I realised that the cells were dying at high...
10 November 2018 8,866 2 View
Dear all, Recently i have a really crazy and brilliant idea to determine if a compound would be able to increase / decrease the motility of a cell type towards another cell type. How should i go...
09 October 2018 2,941 5 View
Dear all, recently i found some strange and uncanny sights in the wells of cells treated with my compound of interest. Apparently when i checked the water solubility of the compound of interest,...
09 October 2018 1,427 3 View
Dear all, i have recently discovered that one of my test compounds is able to promote intercellular aggregation (i.e. the cells are aggregating when treated with the compound). However, i can only...
04 May 2018 7,219 1 View
Dear all, recently i started a cell viability study using presto-blue reagent for the testing. However, i dissolved the drug of interest in sterile water before adding into the wells (96 well...
04 May 2018 8,971 0 View
As above. Thank you :)
10 November 2017 6,237 0 View
10 November 2017 9,256 1 View
10 November 2017 7,693 4 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
03 March 2021 8,237 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
