Dear all, recently i did a scratch test migration assay on my test compounds incubated with my cell line of interest and it appeared that the cell line seem to exhibit signs of increased cell migration. My question is, which proteins/genes related to cell migration/motility should i look at to further expand my current scope of research?
Thank you in advance for your kind responses.
Hey Justin,
You may look for the roles of Matrix Metalloprotease (MMP) -2 and 9 in investigating the observed phenotype, upon your test compounds treatment.
You may refer this paper.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789103/
Dear sir, thank you for your response. Is there any reason why mmp should be considered first among the many proteins involved in cell migration such as moesin?
Dear Justin,
In the case of migration there's many things to look at that listing proteins would be not so helpful. Besides, many of them may have synergism with each other masking the target you are searching for.
Focus on your treatment, what it does. Gather info about the signal pathways it may be interfering with and from there infer possible candidates. Don't worry if you don't find immediate migration effectors in your search, they could be secondary downstream effectors associated with migration. Then you can start testing some of them.
Otherwise, by randomly pinpointing individual targets, you may end up wandering in the dark which surely will become more expensive and time consuming.
I don't know whether you are facing a short or long project, but if you are sure to have a migration phenotype and if you have the resources, I would recommend to do some proteomics on your control and treated samples. Then you can compare by protein ontology among several targets that changed expression pattern after
the treatment. This type of study also helps to better see the "full picture" of what your treatment causes to the cells.
Best,
Dear Dr Sebastian, thank you for your kind response. In that case, which direction or in-vitro assay would you recommend i should proceed if i would like to establish cell migration as the primary mode of a compound in their contribution to a diseased state?
Thank you Dr Sebastian for your response. Kindly ignore my question as i did not see your long answer. Noted with thanks.
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