trying to develop doxorubicin resistant cells. How to store and for how long the doxorubicin solution?
how to count metastatic nodule in slides stained with H&E. different sections have different number of nodules. Should I add all or should I report the section that showed the highest number?
05 March 2024 4,640 2 View
I need doxorubicin resistant breast cancer cells (MDA-MB-231, 4T1 preference). It will take several months to develop the resistant strains. I will be grateful for ever if any of you is willing...
26 June 2023 6,026 1 View
Can anyone tell me what is the luciferin mostly used for in vivo imaging: D-Luciferin potassium salt? or D-Luciferin sodium salt?, who do you buy from?
16 October 2022 513 1 View
I have been trying to proliferate cells for almost 4 weeks culturing 4T1 cells in RPMI 1640 medium supplemented as recommended by the ATCC (10% FBS, 5% antibiotic). Started with a frozen vial...
30 May 2022 7,133 3 View
I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45...
27 September 2021 6,020 0 View
Greetings, I am cultivating Daphnia magna for a while now and after a sudden change in the temperature (to 17 degrees during a weekend) they have not been able to recover. They are still able to...
01 May 2019 8,741 9 View
I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?
13 April 2019 3,424 2 View
I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...
06 November 2017 5,562 3 View
I would like to check for T cell proliferation upon specific stimuli in mouse splenocytes. I know that it is possible to FACS on frozen splenocytes and look at T cell activation therefore I was...
24 February 2016 7,395 0 View
Dear All: Thank you in advance for your help. I am doing my report (systematic review) on this topic, but I would like to centre it in medical and dental postgraduate students. Most of the studies...
30 June 2015 3,030 8 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
thanks
25 June 2024 4,667 0 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
23 June 2024 6,583 2 View
HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...
18 June 2024 5,741 0 View
I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.
10 June 2024 8,347 1 View