Derek Giuliana

5 Questions 4 Answers 0 Followers

Questions related from Derek Giuliana

What concentrations of blasticidin-s have people used when selecting MKN-45 cells?

I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45...

28 September 2021 6,044 0 View

What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

14 April 2019 3,437 2 View

Should I look at BCL-2 in whole cell or subcellular fraction?

I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...

07 November 2017 5,574 3 View

How can I establish good 4T1 tumors in-vivo?

I'm growing 4T1 cells in mice. I'm implanting two contralateral tumors per mouse. I'm having a hard time getting the tumors to grow symmetrically. I understand they won't all be perfect, but I'm...

05 June 2015 9,242 6 View

Protocl for measuring BCL-2 in SK-OV-3 cultured cells?

I'm measuring BCL-2 by R&D's total humanBCL-2 Duo set ELISA. I'm treating cultured SK-OV-3 cells grown in low serum conditions. I'm wringing my hands as I can't show BCL-2 inhibition in the...

01 January 1970 2,348 2 View