How to count metastatic nodule in slide?

More Giuliana D Noratto's questions See All

I am trying to develop doxorubicin resistant cells. How to store and for how long the doxorubicin solution?

trying to develop doxorubicin resistant cells. How to store and for how long the doxorubicin solution?

16 January 2024 7,273 0 View

Could anybody please share doxorubicin resistant breast cancer cells with me?

I need doxorubicin resistant breast cancer cells (MDA-MB-231, 4T1 preference). It will take several months to develop the resistant strains. I will be grateful for ever if any of you is willing...

26 June 2023 6,026 1 View

D-luciferin salt and best supplier?

Can anyone tell me what is the luciferin mostly used for in vivo imaging: D-Luciferin potassium salt? or D-Luciferin sodium salt?, who do you buy from?

16 October 2022 513 1 View

Why my 4T1 breast cancer cells are not growing?

I have been trying to proliferate cells for almost 4 weeks culturing 4T1 cells in RPMI 1640 medium supplemented as recommended by the ATCC (10% FBS, 5% antibiotic). Started with a frozen vial...

30 May 2022 7,133 3 View

What concentrations of blasticidin-s have people used when selecting MKN-45 cells?

I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45...

27 September 2021 6,020 0 View

How to improve a Daphnia culture that is dying (without a clear reason?

Greetings, I am cultivating Daphnia magna for a while now and after a sudden change in the temperature (to 17 degrees during a weekend) they have not been able to recover. They are still able to...

01 May 2019 8,741 9 View

What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

13 April 2019 3,424 2 View

Should I look at BCL-2 in whole cell or subcellular fraction?

I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...

06 November 2017 5,562 3 View

Anyone knows whether is possible to perform an LST on frozen splenocytes?

I would like to check for T cell proliferation upon specific stimuli in mouse splenocytes. I know that it is possible to FACS on frozen splenocytes and look at T cell activation therefore I was...

24 February 2016 7,395 0 View

What kind of interventions on empathy or compassion are effective for health students?

Dear All: Thank you in advance for your help. I am doing my report (systematic review) on this topic, but I would like to centre it in medical and dental postgraduate students. Most of the studies...

30 June 2015 3,030 8 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Bibiana Bernal

are they from a patient? all are they from a experiment, a control case refered to a for example, square or something related to a white control

John Hardy Lockhart

I think it depends on how close those sections are to each other. For example, are the nodules in one slide present in another?

I'd suggest analyzing all of the H&E slides you have from each sample. You can then use the total number or the average across the slides, however, I would advise against just using the highest number in one slide.