How to pack empty column with zinc powder?

How do you pack empty column with zinc powder? This is for the Vitamin K1 analysis using HPLC.

26 October 2020 3,904 3 View

What is the suitable temperature to store Vit A, E, D sample extract extracted from milk powder?

I run the sample preparation using AOAC Vit A,E,D method, that require saponification, concentration , drying using nitrogen gas, reconstitute with methanol. Since the HPLC method development is...

23 October 2020 6,430 4 View

Can a manuscript be removed from a journals' editorial bench without the consent of the author?

Hello, I submitted a manuscript for publication to Journal X in the month of May. A week later the status showed under review. A month after that (June) it showed 1/2 review submitted. Last week I...

15 October 2020 1,995 3 View

How can i recognise a predatory journal?

Hello, I submitted a manuscript for publication in a certain journal and the manuscript was accepted for publication 2 weeks later (from the time of submission) without any review comment. I am...

08 September 2020 4,684 3 View

How do we make multi Fatsa files?

Hi, previously I had used MUMer to run but the input are only able to upload 1 Fasta file. When I test run a reference and the sample it work great but when I combine my sample 1 and sample 2 data...

25 July 2019 3,681 1 View

Why lower molecular weight protein bands disappeared in Tricine SDS PAGE?

Hi, I run this gel using 3 different layers: -the upper layer: 4%T 3%C stacking gel -the middle layer: 10%T 3%C spacer gel (1cm) -the lower layer: 16%T 6%C separating gel -voltage: 60V until the...

17 June 2019 9,711 1 View

Does compensation need to be done on each treatment group to study the effect of treatments on the cell surface marker expression of a cell line?

I would like to investigate the effect of treatments on the cell surface expression of a cell line. Assuming that one treatment upregulates FITC-stained marker and the level of PE-stained marker...

30 August 2016 5,343 3 View

Can laminin-coated culture vessels be reused for multiple cell passaging?

23 February 2016 3,850 3 View

Could we define the cancer stem cell subpopulation within a cancer cell line solely based on its expression of aldehyde dehydrogenase (ALDH)?

Read about distinguishing cancer stem cells (CSCs) from its non-CSC counterpart based on stem cell surface markers, such as CD44, CD24, and CD133, is ambiguous and questionable. Is aldehyde...

13 June 2015 4,194 11 View

Is inconsistency commonly seen in replicates of cell immunophenotyping?

I would like to immunophenotype cancer cell lines based on the expression of cell surface markers. I have done three individual experiments with cells of different passage number, which is at...

13 May 2015 7,490 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

How to inject RNAi into moth larvae?

Hi, I would like a detailed explanation/photos/video How to do it in moth? (I work with Helicoverpa armigera and Spodoptera frugipreda larvae)

04 January 2021 8,194 4 View

Could you suggest me some suitable pesticides for insect larvae in spirulina farm?

Hi everybody , I hope all is well. I've got a question Could you suggest me some suitable pesticides for insect larvae in spirulina farm? Thanks

30 December 2020 2,297 7 View

Santolina species, wax moth and bees?

Expect a savage answer and being reported to ResearchGate Admin if your answer clearly shows you did not read the whole question and you are scamming the points system by answering with an...

12 December 2020 4,413 7 View

Could someone please help me in identification these larvae fish?

I have some images of fish larvae. please guide me in correct identification. thanks

10 December 2020 6,446 3 View

Hello. Is it possible to run very crude samples (grinded larvae) on SDS-gel and have some reasonable results?

In order to investigate if there are any differences on the proteins level between three larvae cultures grown on three different diets (Oat, polyethylene, Polystyrene, we are considering to...

19 November 2020 3,416 3 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

Bloodworms (Chironomus sp) mass production protocol?

Anyone knows an effective protocol to mass produce bloodworms larvae at lab?

26 October 2020 6,998 2 View