How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there any free software or website to analyze the LC-MS/MS data for drug metabolism study?

I have the data for drug metabolism study (biotransformation) using Q-Exactive Orbitrap in switching mode. I would like to know if there is any open source data analysis software or website? I...

09 October 2018 4,970 3 View

Hello community, someone knows about a structural predictor of drug metabolites?

I am working on my thesis work, hepatic biotransformation of some biactive compounds extracted from natural products in rat microsomal fraction (S9), for which I need to know the possible...

02 March 2018 2,940 2 View

Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

15 January 2018 6,790 3 View

Interpolation of product formation in biocatalysis using integrated Michaelis-Menten equation?

I want to interpolate the amount of product formed (as concentration or % of conversion) vs reaction time in a biocatalysis process. The fitting equation should have as (y) the amount of product...

31 December 2017 7,258 3 View

Does ozone layer present in uppermost layer of earth atmosphere?oxygen is below it ? However ozone molecular weight greater than oxygen? Am i Right?

Biotransformation

10 November 2017 9,220 3 View

Can costisone peritendon injection (1cc of Celestone or else) diffuse into a surrounding joint?

For example, does an injection in ITB could be (or will be) be strongly uptaken into the knee joint or an injection for tennis elbow would be uptaken by the elbow joint??? What the capacity of...

07 August 2017 3,197 2 View

Have anyone worked with ttg2A, ttgA invloved in toluene efflux system in pseudomonas putida?

02 March 2017 643 0 View

What is the best method for L-phenylacetylcarbinol(L-PAC) extraction?

10 November 2016 2,767 3 View

Which enzymes I should check in rapeseed pod related with lodging??

07 August 2016 4,598 2 View

No title

Read 2 answers by scientists with 6 recommendations from their colleagues to the question asked by Gregorio Fistetto on Jun 8, 2016

05 June 2016 7,308 3 View