How to pack empty column with zinc powder?

How do you pack empty column with zinc powder? This is for the Vitamin K1 analysis using HPLC.

26 October 2020 3,904 3 View

What is the suitable temperature to store Vit A, E, D sample extract extracted from milk powder?

I run the sample preparation using AOAC Vit A,E,D method, that require saponification, concentration , drying using nitrogen gas, reconstitute with methanol. Since the HPLC method development is...

23 October 2020 6,430 4 View

Can a manuscript be removed from a journals' editorial bench without the consent of the author?

Hello, I submitted a manuscript for publication to Journal X in the month of May. A week later the status showed under review. A month after that (June) it showed 1/2 review submitted. Last week I...

15 October 2020 1,995 3 View

How can i recognise a predatory journal?

Hello, I submitted a manuscript for publication in a certain journal and the manuscript was accepted for publication 2 weeks later (from the time of submission) without any review comment. I am...

08 September 2020 4,684 3 View

How do we make multi Fatsa files?

Hi, previously I had used MUMer to run but the input are only able to upload 1 Fasta file. When I test run a reference and the sample it work great but when I combine my sample 1 and sample 2 data...

25 July 2019 3,681 1 View

Why lower molecular weight protein bands disappeared in Tricine SDS PAGE?

Hi, I run this gel using 3 different layers: -the upper layer: 4%T 3%C stacking gel -the middle layer: 10%T 3%C spacer gel (1cm) -the lower layer: 16%T 6%C separating gel -voltage: 60V until the...

17 June 2019 9,711 1 View

Does compensation need to be done on each treatment group to study the effect of treatments on the cell surface marker expression of a cell line?

I would like to investigate the effect of treatments on the cell surface expression of a cell line. Assuming that one treatment upregulates FITC-stained marker and the level of PE-stained marker...

30 August 2016 5,343 3 View

Can laminin-coated culture vessels be reused for multiple cell passaging?

23 February 2016 3,850 3 View

Could we define the cancer stem cell subpopulation within a cancer cell line solely based on its expression of aldehyde dehydrogenase (ALDH)?

Read about distinguishing cancer stem cells (CSCs) from its non-CSC counterpart based on stem cell surface markers, such as CD44, CD24, and CD133, is ambiguous and questionable. Is aldehyde...

13 June 2015 4,194 11 View

Is inconsistency commonly seen in replicates of cell immunophenotyping?

I would like to immunophenotype cancer cell lines based on the expression of cell surface markers. I have done three individual experiments with cells of different passage number, which is at...

13 May 2015 7,490 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View

Does anyone have a copy of the sTOFHLA?

Peppercorn books is out of business, and that the creator of the test has been dead for over 5 years. I am looking to use this tool to assess literacy in community dwelling seniors as part of my...

25 January 2021 4,061 2 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

Can data collected through participant observation be interpreted through Interpretative phenomenology?

I have just started to read about phenomenology and hence quite a beginner. I have observed hospital interactions between doctors and patients, noted down the conversations, added the non-verbal...

14 January 2021 3,452 3 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse?

Do you think that genetic inheritance or the environment is more influential in the development of substance abuse ?

04 January 2021 1,587 3 View

What is the current status of black female police officers?

I am a doctoral candidate at Walden University. My dissertation is centered on the lived experience of black female police officers and their perspective of excessive use of force administered...

29 December 2020 8,452 1 View

How does one upload a paper in PDF form so that someone who has requested it can get it?

I am quite willing to share it with the public.

14 December 2020 4,590 4 View