How to compute the refraction index for a 79.5GHz (3.8mm) FMCW Radar Instrument?

Dear everyone, I performed some experiements with a FMCW Radar Instrument emitting waves of 3.8mm. I can see some "movements" when I am looking on the relative displacements (+/- 0.15mm) over...

07 January 2021 2,073 6 View

Experience in using the LiDAR of the new iPad Pro for mapping?

Has anyone experience in using the LiDAR of the new iPad and if so, what App have you used? We want to map fault structures or rather fractures of damage zones and I wonder if this can easily be...

01 August 2020 7,473 4 View

Maleimide dye labelling of Ni2+ NTA resin?

Hi. When I purify my His-tagged membrane protein with IMAC, I label the protein (containing a single cysteine) with fluorescein-5-maleimide while the protein is immobilized to the Ni2+ NTA. I...

20 July 2020 2,065 3 View

Is anyone familiar with making a new cell line available for everyone? How to have ATCC or someone else to sell your cell line?

We have developed a new cell line and would like to make it available to everyone. Is anybody familiar with this process? What are the requirements? Are there other companies other than ATCC that...

19 May 2020 1,390 7 View

2-way ANOVA - splitting data or not?

Hi. My question is about 2-way ANOVA (and Freidmans test). I've no problem with the test per se but rather with the dataset in relation to the test(s). My problem is that I've 16 different...

06 April 2020 6,920 4 View

Why information criteria differ so i cant compare arma models?

Hello, i am trying to forecast using eviews the stock price returns of a specific company. I am running automatic arima forecasting so as eviews evaluates and proposes the best model fit...

30 March 2020 9,431 4 View

Paper for using PROCESS for mediation with two independent variables?

14 October 2019 356 3 View

How to stoichiometry calculate this combustion process?

Hi! This one is for you chemists/chemical engineers out there. I have a fuel, tall oil pitch, that I want to burn mixed with ordinary air in an industrial burner for heat generation. The tall...

01 October 2019 5,143 12 View

How to dissolve CaWO4?

Hi, What is the best way to dissolve pure CaWO4? I tried: - conc. Aqua Regia - conc. Aqua Regia + conc. HF - conc. HNO3 + conc. HF - 7N HNO3 - 7N HNO3 + conc HF. Every experiment was for 55...

29 September 2019 5,982 1 View

How can nanobuffering work?

Dear All I am wondering if someone has an good explanation for the "nanobuffering" behavior of pH responsive polymers. Here is some literature on the topic: Increasing Enzyme Cascade Throughput...

28 August 2019 5,254 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Problem with SDS PAGE gel: Can someone help with improper resolving of proteins?

When we cast a 10% SDS PAGE gel, the protein resolution is not sufficient and is similar to an 8% gel. We tried using a freshly prepared acrylamide from a different box, but the problem still...

30 January 2021 901 2 View