How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation or serum 4% after and before treatment of B65.
when i perform in vivo studies with cell lines, I let the cells get to approx 70% confluence if I am going to add treatment for 24 hrs. If I am going to treat my cells for 48 hrs with drugs I let the cells get to approx 50% confluence. Before any drug treatment, I serum starve my cells overnight or approx 12 hrs.
Serum starvation in the normal cells for longer time will leads to autophagy induction.so keep cells in starved conditions as minum as possible before trating with the drug.