Brandon A Kemp

3 Questions 95 Answers 0 Followers

Questions related from Brandon A Kemp

How can I look at 3 kDa protein with Western blot?

I am trying to verify if ghrelin is present in cortical collecting duct cell lines. Ghrelin is approximately 3 kDa and difficult to find on western blot. Does anyone have any suggestions? Thanks!

12 April 2015 3,937 4 View

Does anyone have experience with phospho NKA alpha 1 antibodies in rat kidney tissue?

I am trying to look at phospho ser 23 NKA alpha 1 in rat kidney tissue. I have used antibodies from cell signaling but my band appears around 120 kDa instead of the predicted 100 kDa on the data...

27 January 2015 5,132 2 View

Does anyone know of a proximal tubule specific marker of basolateral membranes?

I am trying to find a specific basolateral membrane marker that is found only in renal proximal tubule cells in rats in vivo. I want to isolate these membranes with an IP and then look at...

23 December 2014 542 2 View