Manchala Nageswar Reddy

3 Questions 41 Answers 0 Followers

Questions related from Manchala Nageswar Reddy

Did anyone work to make a Panther analysis for upregulated genes ?

Here I try to make out Panther analysis for highly expressed genes detected in Realtime PCR analysis? will it be possible? or any method can be suggested to make gene clustering? Thanks in advance.

11 January 2017 4,987 1 View

Will it be possible to see the mutational changes in the dengue viral NS 1 construct by site directed mutagenesis?

I am trying to see the role of DENGUE NS1 trough PCR based mutagenesis approach by mutating some of the gene elements responsible for secretion of the protein into the blood stream. Is it...

15 December 2014 6,777 3 View

Has anyone found the expression of a protein of interest in the mammalian cell lysate by ELISA?

I want to see the protein expression in which the corresponding gene is cloned in a mammalian vector without a secretory signal. I want to see its protein expression in ELISA by using the lysate...

27 November 2014 5,786 4 View