I have an unknown DNA product (about 500bp) which I want to sequence. I cannot insert it into a vector, because I have no vector. The company I do sequence for cannot do it only for one sample. Is there any other solution?
We'd like to produce recombinant proteins using a vector with His-tag in N-terminus. Our gene is inserted after 10-15bp after the His-tag. At the final step of purification and cleavage, how can...
31 December 2015 3,811 9 View
I've submitted a research article that deals with new biomarkers, and is based on samples received from cancer patients. The patients didn't give the blood for this project, however they consented...
09 October 2014 7,150 14 View
I've beem asked to convert some equations from Word to MathType. Unfortunately, I've not the software. Is there anybody that can help? Are there any online tools for such convertions?
08 September 2014 7,028 1 View
I'd like to perform a stable transfection to human cancer cell lines. I performed the transient with pCVMTNT vector, but I want a stable expression of a protein, which I study. The cell lines I'm...
08 September 2014 2,828 5 View
I'd like to study human resistance to alkaloids of the vinca cell line.
07 August 2014 1,337 4 View
After the Nick Translation protocol (abbottmolecular) of my PCR product (325bp product), the electrophoresis data are not good. There have been tested two different periods of time as well two...
05 June 2014 5,867 9 View
I'd like to perform some experiments by applying metronomic chemotherapy with 5FU and Oxaliplatin in colorectal cancer cell lines. Has anyone used a particular concentration for this application?
02 March 2014 1,099 2 View
I'd like to design a complete protocol for expressing protein in mammalians by using plasmid expression vectors. However, I've never dealt with culture of competent cells, so I'm not familiar with...
02 March 2014 7,190 5 View
I'd like to design a probe for FISH. The area is non-coding, but the sequence is known. The size of the probe should be 500-1500bp. Do I need to use DOP PCR with the desirable primer? Does anyone...
31 December 2013 632 6 View
We would like to analyze the data from antibody array after scanning.
11 December 2013 9,086 1 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.
21 February 2021 6,523 6 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View