12 December 2013 1 9K Report

We would like to analyze the data from antibody array after scanning.

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How to remove the aminoacids after the His-tag and before my protein?

We'd like to produce recombinant proteins using a vector with His-tag in N-terminus. Our gene is inserted after 10-15bp after the His-tag. At the final step of purification and cleavage, how can...

31 December 2015 3,912 9 View

When is an ethical clarification needed in research experiments?

I've submitted a research article that deals with new biomarkers, and is based on samples received from cancer patients. The patients didn't give the blood for this project, however they consented...

09 October 2014 7,266 14 View

Does anyone uses MathType?

I've beem asked to convert some equations from Word to MathType. Unfortunately, I've not the software. Is there anybody that can help? Are there any online tools for such convertions?

08 September 2014 7,144 1 View

Which is an appropriate vector for stable transfection of human cancer cell lines?

I'd like to perform a stable transfection to human cancer cell lines. I performed the transient with pCVMTNT vector, but I want a stable expression of a protein, which I study. The cell lines I'm...

08 September 2014 2,933 5 View

Does anybody know any cell line resistant to alkaloids of vinca and specially on vincristine?

I'd like to study human resistance to alkaloids of the vinca cell line.

07 August 2014 1,451 4 View

How can I increase the efficiency of Nick Translation for preparing a FISH probe?

After the Nick Translation protocol (abbottmolecular) of my PCR product (325bp product), the electrophoresis data are not good. There have been tested two different periods of time as well two...

05 June 2014 5,989 9 View

What dose of metronomic chemotherapy should I use for my cancer cell lines?

I'd like to perform some experiments by applying metronomic chemotherapy with 5FU and Oxaliplatin in colorectal cancer cell lines. Has anyone used a particular concentration for this application?

02 March 2014 1,204 2 View

Can anyone help with protein expression in mammalian cells?

I'd like to design a complete protocol for expressing protein in mammalians by using plasmid expression vectors. However, I've never dealt with culture of competent cells, so I'm not familiar with...

02 March 2014 7,296 5 View

Can anyone help with probes for FISH?

I'd like to design a probe for FISH. The area is non-coding, but the sequence is known. The size of the probe should be 500-1500bp. Do I need to use DOP PCR with the desirable primer? Does anyone...

31 December 2013 753 6 View

Housekeeping gene for gene expression analysis?

I'm using 18SrRNA and β-Actin as housekeeping genes in my qPCR experiments. GAPDH and HPRT1 have higher Ct. However in many samples (CTCs), the 18SrRNA seems to be more accurate.

08 September 2013 9,628 9 View

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