Seeding density of adherent cells in 48 well plate for 7-day DNA quantification?

13 May 2016 1 5K Report

Does anyone have # of adherent cells to seed in 48 well for 7-day DNA quantification assay to study cell proliferation? I would imaging the seeding # will be slightly different from absorbance-based proliferation assays?

Thanks in advance,

Subrata Pore

It depends upon the type of cells and their proliferation rates. You could try to seed around 5000 cells per well in 48 well plate for 7 days for imaging.

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