Sample dispersion between the stacking and resolving gel on SDS PAGE

14 June 2013 1 3K Report

I just ran a and SDS PAGE with 12% resolving gel and 4% stacking gel. The problem is that as soon as I turned on the electricity (180V), the samples moved down the stacking gel in a wiggly manner. Then when they reached the line between the stacking and resolving gels, the samples dispersed. I continued running the gel but after the staining I did not get any bands except for my standard ladder. Has anyone experience such a thing before?

Evgeny Klimuk

Dear Nanda,

Are you sure that you have proteins in samples? Also, does your ladder look as usual? And finally, what is the size of your gel (180V for stacking gel sounds scary)?

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Science method

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