8 Questions 14 Answers 0 Followers
Questions related from Nanda Aung
Does anyone know growth rate of E. coli in davis minimal 25 medium (DM25)? I have been growing the cells for 24 hours and cells are not growing well.
12 January 2015 8,915 0 View
I want to perform a DNA fragmentation apoptosis assay from whole organism DNA prep of Aedes aegypti mosquito. Has anyone performed a similar procedure before even in nematodes or larvae of other...
26 November 2013 9,426 15 View
I have never done a qPCR experiment before so I need help with designing one. The attached is the layout of a 48-well plate that I have designed. I am measuring mRNA expression during a 48-hour...
17 September 2013 8,849 6 View
Does anyone have SDS based DNA isolation protocol from Aedes aegypti mosquito larvae or larvae of any related organisms? I have to isolate DNA from freshly emerged mosquito larvae and older. I...
10 September 2013 1,414 1 View
I need to size dsRNA on agarose gel. I am wondering if I can use a DNA ladder. I was looking at the dsRNA ladder from NEB but the largest band is only 500 bp. However, my gene is supposed to be...
04 September 2013 868 1 View
I just ran a and SDS PAGE with 12% resolving gel and 4% stacking gel. The problem is that as soon as I turned on the electricity (180V), the samples moved down the stacking gel in a wiggly manner....
14 June 2013 3,451 1 View
I am designing an RNAi experiment. I want to observe gene expression in different samples. Would you recommend Taqman or SYBR green assay? I am convinced that SYBR Green assay would be...
03 June 2013 5,218 56 View
I am trying to align a group of proteins. One of the members has its crystal structure published. Is there any multiple sequence alignment program (preferably free) that can align amino acid...
30 May 2013 2,959 6 View
