Ayman Khalifa

14 Questions 9 Answers 0 Followers

Questions related from Ayman Khalifa

Could anti guina pig and anti mice secondary antibody cross react together?

If I used primary guinea pig and mice antibody, could there secondary antibody interact with each other?

20 December 2021 2,880 2 View

How to keep cells active after sorting with FACS?

Hi colleagues, I have a problem with my sorting cells. I isolated human keratinocytes from epidermal skin and I mark them with CD44 and ALDH to sort stem cells from them. The time of staining...

29 November 2021 5,154 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

17 February 2021 8,533 2 View

RTX vs Capsaicin?

Did any body know the differences between Resinferatoxin and capsaicin? Both are used to denervate the sensory nerves .

07 August 2019 1,832 1 View

How to dissolve RTX?

I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...

18 June 2019 619 2 View

What are signs of sympatheic denervation in mice?

I would like to denervate mice skin sympathetically and I will use 6-OHDA. I will be appreciated if you help me to know how can I be sure that the nerves are removed. i found that it can be known...

17 June 2019 6,184 0 View

How can we evaluate physically the effect of Sympathetic denervation in mice?

I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days

29 April 2019 932 3 View

How many days I should wait till be sure that the mice denervation done?

Id like to make symathectomy and sensory denervation in mice skin I will use subcutaneous injection of Resinferatoxin for sensory denervation and interperitoneal injection of 6hydroxydopamine for...

29 April 2019 803 0 View

Could any one kindly please explain to me how to prepare the dilution for my primers?

My primer has 95.05 ug, 3.25 OD, 6124.99 MWT and 15.51 nmol The initial conc we need 10 uM The final conc 5 uM what I understand from online that I will prepare a stock soln first by adding...

20 August 2018 3,483 1 View

Did any body know how to use Baxter software to draw lineage trees?

I'd like to draw cell lineage tress and I found Baxter program do it well but it based on algorithms equations and i cant get it well. Hope any body could kindly help me

18 August 2018 9,851 0 View

Mouse recombinant sonic hedgehog? What is the best way to inject this little dose?

Hello every body, Id like to inject mouse recombinant sonic hedgehog subcutaneously in mouse and the dose will be 1 mg / kg (0.02 mg for 20 gm mice)my question is What is the best way to inject...

06 July 2018 9,225 1 View

Sonic hedgehog in mice?

Hello every body, could any body kindly support me about the data of the subcutaneous injection of Sonic hedgehog to mice to improve wound healing what is the recommended dose and the protocol...

20 June 2018 6,790 2 View

Is the dose injected to the rats same as the dose injected to the mice or it should be different?

I am going to use a chemical and I found its dose to the rats and it is not clear for the mouse can I use the same dose as it depends on weight?

05 June 2018 6,183 3 View

Alignment of my RNA seq data

Hello everyone, I'm currently working on analyzing RNA seq data and downloaded a fastq file from the NCBI website for practice. After using trimmomatic to trim my data, I faced challenges while...

01 January 1970 8,310 4 View