15 Questions 10 Answers 0 Followers
Questions related from Ayman Khalifa
If I used primary guinea pig and mice antibody, could there secondary antibody interact with each other?
20 December 2021 2,948 2 View
Hi colleagues, I have a problem with my sorting cells. I isolated human keratinocytes from epidermal skin and I mark them with CD44 and ALDH to sort stem cells from them. The time of staining...
29 November 2021 5,209 3 View
Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...
17 February 2021 8,637 2 View
Did any body know the differences between Resinferatoxin and capsaicin? Both are used to denervate the sensory nerves .
07 August 2019 1,899 1 View
I am gonna use Resiniferatoxin (RTX) in my experiment found that it is a powder(1 &5 mg) that needed to be dissolved in a vehicle to be allowed for injection when I checked some papers I...
18 June 2019 673 2 View
I would like to denervate mice skin sympathetically and I will use 6-OHDA. I will be appreciated if you help me to know how can I be sure that the nerves are removed. i found that it can be known...
17 June 2019 6,236 0 View
I used 6-OH-dopamine to induce sympathetic denervation in mice How long I should use it Is their any clear signs for denervation or as reviews mentioned to use it for 7 to 14 days
29 April 2019 997 3 View
Id like to make symathectomy and sensory denervation in mice skin I will use subcutaneous injection of Resinferatoxin for sensory denervation and interperitoneal injection of 6hydroxydopamine for...
29 April 2019 854 0 View
My primer has 95.05 ug, 3.25 OD, 6124.99 MWT and 15.51 nmol The initial conc we need 10 uM The final conc 5 uM what I understand from online that I will prepare a stock soln first by adding...
20 August 2018 3,533 1 View
I'd like to draw cell lineage tress and I found Baxter program do it well but it based on algorithms equations and i cant get it well. Hope any body could kindly help me
18 August 2018 9,914 0 View
How can we draw the lineage tress ? did we use a certain software or just using excel and word aatached is a sample fig from a paper to Amit Roshan et al., " Human keratinocytes have two...
09 August 2018 4,483 0 View
Hello every body, Id like to inject mouse recombinant sonic hedgehog subcutaneously in mouse and the dose will be 1 mg / kg (0.02 mg for 20 gm mice)my question is What is the best way to inject...
06 July 2018 9,300 1 View
Hello every body, could any body kindly support me about the data of the subcutaneous injection of Sonic hedgehog to mice to improve wound healing what is the recommended dose and the protocol...
20 June 2018 6,856 2 View
I am going to use a chemical and I found its dose to the rats and it is not clear for the mouse can I use the same dose as it depends on weight?
05 June 2018 6,228 3 View
Hello everyone, I'm currently working on analyzing RNA seq data and downloaded a fastq file from the NCBI website for practice. After using trimmomatic to trim my data, I faced challenges while...
01 January 1970 8,361 4 View
