How to (seriously) read a scientific paper?

02 March 2016 1,705 2 View

Is there any measurable benefit of publishing as Preprints?

What is possible legitimacy of publishing in such a way. Does it have any same value as that published in Journals or is it just to show that one has done some work ahead of any other in that...

01 February 2016 3,826 4 View

Does changing in vol of DMSO, Polymerase buffer and or pfu solution affect bands appearance?

I have the problem of appearance of nan-specific bands when I change the volume of DMSO, Pfu, Polymerase buffer and or DW in sample solution for PCR. I also change the annealing temperature due to...

09 October 2015 2,784 5 View

What is meant by a solar hybrid water treatment system or device?

08 September 2014 1,414 7 View

HPLC-UV method for analysis of Trypsin and/or Chymotrypsin?

06 July 2014 5,142 1 View

What is the usefulness of HPLC method development software?

Talking with colleagues, it seems that the possible usefulness of an HPLC method development software repeatedly comes into the analyst's mind during the development of a tough HPLC/UPLC assay....

03 April 2014 6,574 1 View

What would be the best ratio of AgNO3:PVP+PEG?

What would be the best ratio of AgNO3:PVP+PEG, so as to have complete reduction of my precursor and also get 100% capped NPs?

31 December 2013 2,696 2 View

How can I get the double divisor ratio spectra?

How can I get the double divisor ratio spectra method for ternary components analysis, while using double beam 1650PC Shimadzu spectrophotometer, I can not understand the methods produced in few...

31 December 2013 2,662 1 View

Why is the reduction process in forced degradation studies for API/drug product not performed?

In forced degradation studies for API/drug product we are performing hydrolysis (acid, base & water), Oxidation, Photolysis and thermal degradation conditions. But why we are not performing reduction.

31 December 2013 9,249 5 View

What is meant by different colours of Silver NPs?

I have developed Silver NPs with PEG-PVP combinations but sometimes I get various coloration changes, I do not understand which colour is the endpoint of my reaction between precursor and reducing...

31 December 2013 9,878 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View