19 Questions 18 Answers 0 Followers
Questions related from Harshad Paithankar
My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...
08 August 2015 1,007 13 View
I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...
02 February 2015 5,604 3 View
How do I convert the fret Eapp data to the dwell time?
11 November 2014 4,860 2 View
I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...
09 September 2014 6,035 8 View
Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...
09 September 2014 7,952 7 View
Is there any comparative study of protein purification or separation by using reverse phase HPLC and fast protein liquid chromatography (FPLC)? What are the criteria for separation of protein by...
03 March 2014 2,297 1 View
I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.
07 July 2013 598 3 View
I am interested for the UV spectra of vitamins.
03 March 2013 9,695 3 View
Is there any way other than prediction from signal to noise to ratio? In case of s/n, the s/n for the peak near its base should be considered or the ratio should be calculated from the base line...
02 February 2013 5,987 17 View
for example, ODS, synchronus, hypersil, nucleosil, etc.
01 January 2013 679 10 View
I have collected the physical and chemical data from three batches. Is this sufficient for the development of in house standards? how do I proceed with the data available?
12 December 2012 2,464 0 View
I want to analyse the quantitatively or semi-quantitatively chromium, copper, tin.
11 November 2012 3,639 6 View
Is there any variable in UV detectors, which give equal or better scanning speed than PDA detectors? What is the scanning speed of the different systems?
11 November 2012 5,499 4 View
Mainly lopinavir and ritonavir
07 July 2012 6,869 3 View
Analysis of lc-ms data.
05 May 2012 7,314 19 View
I am interested in data acquisition in jasco hplc.
05 May 2012 3,969 7 View
I am using an HPLC with UV detector made by JASCO.
05 May 2012 3,752 1 View
I am using jasco hplc with uv detector and borwin software.
04 April 2012 2,891 6 View
Is it possible to get various softwares or at least their user manuals to understand them? Is it possible to download such information from anywhere?
03 March 2012 9,457 14 View
