How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

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02 December 2020 7,293 2 View

What is the physical meaning of the cross term of the index performance of LQR ?

Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...

02 August 2020 1,780 1 View

Can anyone help me about probability sampling?

Now I am doing my DBA research, the whole population are the public university teachers in one of Chinese 's province, there are 22 universities. and I want 400 samplings, the total population is...

23 February 2020 248 3 View

On-market phage resistant E.coli strains for pr expression?

ER2566 by NEB is what we are utilizing for pr expression, with merits of fhuA2 mutation. Apparently its resistance spectrum is not wide enough to combat all kinds of bacteriophages we encounter,...

28 December 2019 3,211 3 View

3'deoxy ribonucleotide available?

Hi, I wonder if people know 3'deoxy ribonucleotide (i.e. with 2'OH) is commercially available? I didn't find any in the modified bases product section in IDT, thermo, or sigma. I'd like to use...

14 October 2019 8,376 3 View

Has a researcher done or is doing research on the photoinitiator tetraethylmethane ketone?

Dear friends, why use tetraethylmethane ketone as photoinitiator (5wt% of photomonomer added), triethanolamine as co-initiator, UV-induced (light wavelength 365nm) acrylamide double bond...

17 April 2019 9,857 1 View

How to quantify amyloid load of IHC staining images with Image J?

Hi, everyone, I would like to quantify the Abeta load of my IHC staining images. How should I do it? someone use plaque area %, someone use plaque numbers, which one is better? And can I do it...

15 February 2019 2,109 1 View

How many dose can we use in radiotherapy?

Hi We designed a radiotherapy study, in this experiment we used hypofraction therapy that 4 Gy per day treated local tumor and gave 4 days, the total dose is 16 Gy. However, we observed 3 of 6...

11 May 2018 6,966 3 View

How to compare effect size between two items?

Dear all experts, I'm currently doing a fMRI study and compare two groups in two conditions (switch and non-switch), and wondering if they have differences in one ROI (region of...

18 April 2018 2,789 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View