How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

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02 December 2020 7,293 2 View

What is the physical meaning of the cross term of the index performance of LQR ?

Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...

02 August 2020 1,780 1 View

Can anyone help me about probability sampling?

Now I am doing my DBA research, the whole population are the public university teachers in one of Chinese 's province, there are 22 universities. and I want 400 samplings, the total population is...

23 February 2020 248 3 View

On-market phage resistant E.coli strains for pr expression?

ER2566 by NEB is what we are utilizing for pr expression, with merits of fhuA2 mutation. Apparently its resistance spectrum is not wide enough to combat all kinds of bacteriophages we encounter,...

28 December 2019 3,211 3 View

3'deoxy ribonucleotide available?

Hi, I wonder if people know 3'deoxy ribonucleotide (i.e. with 2'OH) is commercially available? I didn't find any in the modified bases product section in IDT, thermo, or sigma. I'd like to use...

14 October 2019 8,376 3 View

Has a researcher done or is doing research on the photoinitiator tetraethylmethane ketone?

Dear friends, why use tetraethylmethane ketone as photoinitiator (5wt% of photomonomer added), triethanolamine as co-initiator, UV-induced (light wavelength 365nm) acrylamide double bond...

17 April 2019 9,857 1 View

How to quantify amyloid load of IHC staining images with Image J?

Hi, everyone, I would like to quantify the Abeta load of my IHC staining images. How should I do it? someone use plaque area %, someone use plaque numbers, which one is better? And can I do it...

15 February 2019 2,109 1 View

How many dose can we use in radiotherapy?

Hi We designed a radiotherapy study, in this experiment we used hypofraction therapy that 4 Gy per day treated local tumor and gave 4 days, the total dose is 16 Gy. However, we observed 3 of 6...

11 May 2018 6,966 3 View

How to compare effect size between two items?

Dear all experts, I'm currently doing a fMRI study and compare two groups in two conditions (switch and non-switch), and wondering if they have differences in one ROI (region of...

18 April 2018 2,789 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View