i found the content of dry weight of euglena gracilis under mixtrophic is much higher than than under heterotrophic. almost 2-fold. is that right?or something wrong happened?
Technically, mixotrophic growth should have higher biomass concentration than that heterotrophic growth.
Mixotrophic = autotrophic + heterotrophic
Mixotrophic cultivation of microalgae involves the simultaneous utilization of assimilated CO2 and organic carbon to produce biomass and metabolites via both respiratory and photosynthetic routes [1]. This allows microalgae to utilize both inorganic and organic carbon sources, such as glucose, which can enhance biomass yield [2]. In comparison, heterotrophic cultures grow on organic carbon in the absence of light via combined respiratory and fermentative routes [1]. A study on the optimization of culture media for enhanced microalgae production found that mixotrophic optimized media resulted in maxima of biomass and lipid in comparison to that of other cultivation conditions media [1].
Reference:
[1] Photoautotrophic, mixotrophic, and heterotrophic culture media optimization for enhanced microalgae production - ScienceDirect