RNA is analyzed for the location of hairpin folds. sequences could form a minihairpin?

More Ashu Srivastav's questions See All

Can anyone explains about this question related to this manuscript?

Dear All, I need your help asap. I have gone through this paper and want to understand in-depth and resolve this question. Please have a look at it and give me a possible understanding. I have to...

31 December 2019 7,485 1 View

Looking for Post Doctorate Fellow Position?

I am a PhD Biotechnology student. I want to do PDF. If anyone know about any position or any scientist is having any vacancy please let me know. Thank you

04 May 2019 8,211 3 View

PLGA np size range from 80-220nm effect on cells?

We prepared PLGA np of different sizes ranging from 80, 100, 120,...., 200, 220nm. Do you think it has an effect on cell lines. Cellular uptake behaviour internalization and MTT assay.??

02 March 2018 2,296 4 View

Drug loading efficiency methodology?

Can anyone suggest about the drug loading efficiency to the nanoparticles and nanotubes???Possible method for its release mechanism

03 April 2016 2,803 7 View

Neuronal regeneration mechanism for CNS: cell type selection for the model?

Can anyone suggest about the 3 D brain on chip development for CNS regeneration study..What are the cell types which we should mainly focus or cell lines. Else directly neural progenitor cell will...

01 February 2016 3,847 2 View

Fluorescent dye loaded with nanotube/nanoparticle can be calculated???

If I am loading fluorescent dye of 200ul and nanoparticle of 200ul both are in PBS. The dye conc. is (20 mg of dye in 1 ml of PBS) with a nanoparticle concentration of 3.5 mg/ml. How I will get to...

31 December 2015 9,206 4 View

Can anyone tell me where Image Flow Cytometers are installed in India and where they are running experiments?

Can anybody suggest where I can conduct flow cytometry in India? Where are labs installed and experiments on running?

01 February 2015 1,377 8 View

Can anyone comment or advise about Eudragit S100 fluorescent tagging dye?

I wanted to tag Eudragit S100 for the cellular uptake studies. As per some of the papers it is tagged with Rhodamin B or Nile Red (567/580nm). But I have a limitation for excitation laser. I can...

01 February 2015 9,175 1 View

How to confirm our tagging is proper for Nanoparticle labelling checking?

Can Anyone suggest me about Eudragit S100 tagging with Rhodamine B fluorescent dye. How to confirm that our tagging is proper?

01 February 2015 5,365 3 View

Is this work can be possible and good???

"ABCA2 transporter mutant SP1 transcription factor gene have altered distribution of biogenic amine in human brain"

06 July 2014 3,881 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View