Can anyone explains about this question related to this manuscript?

Dear All, I need your help asap. I have gone through this paper and want to understand in-depth and resolve this question. Please have a look at it and give me a possible understanding. I have to...

31 December 2019 7,370 1 View

Looking for Post Doctorate Fellow Position?

I am a PhD Biotechnology student. I want to do PDF. If anyone know about any position or any scientist is having any vacancy please let me know. Thank you

04 May 2019 8,120 3 View

PLGA np size range from 80-220nm effect on cells?

We prepared PLGA np of different sizes ranging from 80, 100, 120,...., 200, 220nm. Do you think it has an effect on cell lines. Cellular uptake behaviour internalization and MTT assay.??

02 March 2018 2,188 4 View

Drug loading efficiency methodology?

Can anyone suggest about the drug loading efficiency to the nanoparticles and nanotubes???Possible method for its release mechanism

03 April 2016 2,664 7 View

Neuronal regeneration mechanism for CNS: cell type selection for the model?

Can anyone suggest about the 3 D brain on chip development for CNS regeneration study..What are the cell types which we should mainly focus or cell lines. Else directly neural progenitor cell will...

01 February 2016 3,724 2 View

Fluorescent dye loaded with nanotube/nanoparticle can be calculated???

If I am loading fluorescent dye of 200ul and nanoparticle of 200ul both are in PBS. The dye conc. is (20 mg of dye in 1 ml of PBS) with a nanoparticle concentration of 3.5 mg/ml. How I will get to...

31 December 2015 9,088 4 View

Can anyone tell me where Image Flow Cytometers are installed in India and where they are running experiments?

01 February 2015 1,270 8 View

Can anyone comment or advise about Eudragit S100 fluorescent tagging dye?

I wanted to tag Eudragit S100 for the cellular uptake studies. As per some of the papers it is tagged with Rhodamin B or Nile Red (567/580nm). But I have a limitation for excitation laser. I can...

01 February 2015 9,062 1 View

How to confirm our tagging is proper for Nanoparticle labelling checking?

Can Anyone suggest me about Eudragit S100 tagging with Rhodamine B fluorescent dye. How to confirm that our tagging is proper?

01 February 2015 5,259 3 View

Is this work can be possible and good???

"ABCA2 transporter mutant SP1 transcription factor gene have altered distribution of biogenic amine in human brain"

06 July 2014 3,766 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View