Can anyone explains about this question related to this manuscript?

Dear All, I need your help asap. I have gone through this paper and want to understand in-depth and resolve this question. Please have a look at it and give me a possible understanding. I have to...

31 December 2019 7,370 1 View

Looking for Post Doctorate Fellow Position?

I am a PhD Biotechnology student. I want to do PDF. If anyone know about any position or any scientist is having any vacancy please let me know. Thank you

04 May 2019 8,120 3 View

PLGA np size range from 80-220nm effect on cells?

We prepared PLGA np of different sizes ranging from 80, 100, 120,...., 200, 220nm. Do you think it has an effect on cell lines. Cellular uptake behaviour internalization and MTT assay.??

02 March 2018 2,188 4 View

Drug loading efficiency methodology?

Can anyone suggest about the drug loading efficiency to the nanoparticles and nanotubes???Possible method for its release mechanism

03 April 2016 2,664 7 View

Neuronal regeneration mechanism for CNS: cell type selection for the model?

Can anyone suggest about the 3 D brain on chip development for CNS regeneration study..What are the cell types which we should mainly focus or cell lines. Else directly neural progenitor cell will...

01 February 2016 3,724 2 View

Fluorescent dye loaded with nanotube/nanoparticle can be calculated???

If I am loading fluorescent dye of 200ul and nanoparticle of 200ul both are in PBS. The dye conc. is (20 mg of dye in 1 ml of PBS) with a nanoparticle concentration of 3.5 mg/ml. How I will get to...

31 December 2015 9,088 4 View

Can anyone tell me where Image Flow Cytometers are installed in India and where they are running experiments?

01 February 2015 1,270 8 View

Can anyone comment or advise about Eudragit S100 fluorescent tagging dye?

I wanted to tag Eudragit S100 for the cellular uptake studies. As per some of the papers it is tagged with Rhodamin B or Nile Red (567/580nm). But I have a limitation for excitation laser. I can...

01 February 2015 9,062 1 View

Is this work can be possible and good???

"ABCA2 transporter mutant SP1 transcription factor gene have altered distribution of biogenic amine in human brain"

06 July 2014 3,766 0 View

RNA is analyzed for the location of hairpin folds. sequences could form a minihairpin?

(A) AGGUUUCCU (B) AAAAAAAAA (C) AGGUUUGGA (D) AGGUUUAGG

03 April 2013 5,905 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View