Can anyone explains about this question related to this manuscript?

Dear All, I need your help asap. I have gone through this paper and want to understand in-depth and resolve this question. Please have a look at it and give me a possible understanding. I have to...

31 December 2019 7,370 1 View

Looking for Post Doctorate Fellow Position?

I am a PhD Biotechnology student. I want to do PDF. If anyone know about any position or any scientist is having any vacancy please let me know. Thank you

04 May 2019 8,120 3 View

PLGA np size range from 80-220nm effect on cells?

We prepared PLGA np of different sizes ranging from 80, 100, 120,...., 200, 220nm. Do you think it has an effect on cell lines. Cellular uptake behaviour internalization and MTT assay.??

02 March 2018 2,188 4 View

Drug loading efficiency methodology?

Can anyone suggest about the drug loading efficiency to the nanoparticles and nanotubes???Possible method for its release mechanism

03 April 2016 2,664 7 View

Neuronal regeneration mechanism for CNS: cell type selection for the model?

Can anyone suggest about the 3 D brain on chip development for CNS regeneration study..What are the cell types which we should mainly focus or cell lines. Else directly neural progenitor cell will...

01 February 2016 3,724 2 View

Fluorescent dye loaded with nanotube/nanoparticle can be calculated???

If I am loading fluorescent dye of 200ul and nanoparticle of 200ul both are in PBS. The dye conc. is (20 mg of dye in 1 ml of PBS) with a nanoparticle concentration of 3.5 mg/ml. How I will get to...

31 December 2015 9,088 4 View

Can anyone comment or advise about Eudragit S100 fluorescent tagging dye?

I wanted to tag Eudragit S100 for the cellular uptake studies. As per some of the papers it is tagged with Rhodamin B or Nile Red (567/580nm). But I have a limitation for excitation laser. I can...

01 February 2015 9,062 1 View

How to confirm our tagging is proper for Nanoparticle labelling checking?

Can Anyone suggest me about Eudragit S100 tagging with Rhodamine B fluorescent dye. How to confirm that our tagging is proper?

01 February 2015 5,259 3 View

Is this work can be possible and good???

"ABCA2 transporter mutant SP1 transcription factor gene have altered distribution of biogenic amine in human brain"

06 July 2014 3,766 0 View

RNA is analyzed for the location of hairpin folds. sequences could form a minihairpin?

(A) AGGUUUCCU (B) AAAAAAAAA (C) AGGUUUGGA (D) AGGUUUAGG

03 April 2013 5,905 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View