I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide.
So, I know that the molecular weight of my peptide is 8770 da, and that it has 75 aa. I thought I could find a 'similar-ish' protein and ELISA pair and do the kinetic validation with that (based on the fact that its mostly size that determines release from the hydrogel). Is there an easy way to find out whether there are similar proteins? I have used PROTEIN via NCBI, and the Similar Protein search and this only pulls up the similar family members, for which there is also no antibody/ELISA. I have also used slighly wider search criteria in PROTEIN and tried to manually wade though the hits, but no luck yet on a protein that I can purchase off the shelf, and that has an available ELISA.
THANKS for getting this far! :-)
Insulin is a bit smaller at 5.7 kDa. There are ELISA kits available.
Here is a list of human peptide hormones. There are probably ELISAs for most of them. You will have to look each one up to find the molecular weights.
https://en.wikipedia.org/wiki/List_of_human_hormones#Peptide
Hi Bobbi,
you may try ubiquitin, which is 76 residues and 8565 Daltons.
You may check for ELISA ubiquitin antibodies at Santa Cruz:
https://www.scbt.com/scbt/search?Ntt=Ubiquitin
It is also available as recombinant expressed with Flag or Histidine tags (around 10 kDa) at Sigma:
https://www.sigmaaldrich.com/catalog/search?term=ubiquitin&interface=All&N=0&mode=partialmax&lang=pt®ion=BR&focus=product
I knew that there were people about with this knowledge, and I thank you both very much for taking the time to share it.
Cheers!
B.
I would suggest:
Try using a human or mouse chemokine...This would be helpful because:
1-Most chemokines are of ~ 8 kDa (e.g. CXCL10, CCL3)
2-They are available for purchase from multiple companies (e.g. RND, Peprotech, Biolegend), so would be fast to get the assay optimized.
3-There are lots of commercially-available ELISAs for them, and a good number of antibodies, so you can even design a custom ELISA if you so wish.
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