Reverse engineering a protein - kind of?

I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide. So, I know that the molecular weight of my...

09 October 2018 6,332 4 View

Paper using PSD95 antibody in a Dlg4 KO mouse ?

I have been asked by a reviewer to justify the specificity of a Dlg4 antibody - MA1-045 (6G6-1C9) from Thermo (previously millipore). Does anyone know in what paper someone has done the control...

09 October 2016 5,253 3 View

Does anyone know where I can get the list of genes which are known downstream targets for the transcription factor CEBPB?

Using Enricher I can see that my gene set contains 235/3478 genes known to be targets of CEBPB via the Transfac/Jasper tab. Does anyone know where i could get the list of all the CEBPB genes so...

01 February 2016 9,597 3 View

Does anyone know of research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses?

10 November 2015 9,865 4 View

Anti-Iba1 antibody (ab5076) : Does anyone have any experience with it in mouse or monkey?

We are looking for a good alternative to the Rabbit-Anti-Iba1 from Wako as although it it great it limits the use of other rabbit antibodies for double labelling. Does anyone have any experience...

08 September 2013 7,652 14 View

Stability of estradiol in PBS frozen at -20 - can anyone help?

Would I expect there to be a significant loss of bioactivity of E4 diluted in PBS and then frozen once before use? I would prefer to make a batch and then aliquot and store, as opposed to making...

08 September 2013 8,288 8 View

Microglial Primary Culture- one brain at a time?

We have mixed genotype litters of pups that we would like to culture, but it is difficult to do the genotyping before culturing the microglia (either E19 or P0 pups) and we were wondering how...

06 July 2013 2,744 2 View

Is it beneficial to use ELISA to quantify p53 levels in cultured cell fractions?

ELISA and western blots are only as good as the antibodies used to make them - but despite a plethora of published westerns for measuring p53 there are very few reports using ELISA. I have...

10 November 2012 8,518 15 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View