Reverse engineering a protein - kind of?

I want to bind a peptide to a hydrogel and test the release kinetics over time using an ELISA. BUT, there is no antibody/ELISA available for my peptide. So, I know that the molecular weight of my...

09 October 2018 6,332 4 View

Thalamic photothrombotic stroke - intersecting lasers?

Is it possible to use two intersecting lasers whose field strength is added at their point of intersection to activate a thrombotic dye? I would like to be able to target more than just the cortex...

10 November 2017 9,391 0 View

Does anyone know where I can get the list of genes which are known downstream targets for the transcription factor CEBPB?

Using Enricher I can see that my gene set contains 235/3478 genes known to be targets of CEBPB via the Transfac/Jasper tab. Does anyone know where i could get the list of all the CEBPB genes so...

01 February 2016 9,597 3 View

Does anyone know of research looking at the effects of repeated viral infection in first few weeks of life in a mouse to model childhood illnesses?

10 November 2015 9,865 4 View

Anti-Iba1 antibody (ab5076) : Does anyone have any experience with it in mouse or monkey?

We are looking for a good alternative to the Rabbit-Anti-Iba1 from Wako as although it it great it limits the use of other rabbit antibodies for double labelling. Does anyone have any experience...

08 September 2013 7,652 14 View

Stability of estradiol in PBS frozen at -20 - can anyone help?

Would I expect there to be a significant loss of bioactivity of E4 diluted in PBS and then frozen once before use? I would prefer to make a batch and then aliquot and store, as opposed to making...

08 September 2013 8,288 8 View

Microglial Primary Culture- one brain at a time?

We have mixed genotype litters of pups that we would like to culture, but it is difficult to do the genotyping before culturing the microglia (either E19 or P0 pups) and we were wondering how...

06 July 2013 2,744 2 View

Is it beneficial to use ELISA to quantify p53 levels in cultured cell fractions?

ELISA and western blots are only as good as the antibodies used to make them - but despite a plethora of published westerns for measuring p53 there are very few reports using ELISA. I have...

10 November 2012 8,518 15 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View