Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

Can the content of my research section be shown on the front page?

no details needed

26 December 2020 9,690 3 View

How do you transmit and evaluate ethics and moral values in you teaching activity?

Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?

23 December 2020 5,058 7 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

Does anyone know of cases of complete eye evulsion (eyes jumping out of their sockets) in ancient or medieval sources?

01 November 2020 1,234 3 View

If an observed d falls beyond the upper limit in equivalence testing, what does that mean?

I have been taking your Coursera Classes and reading some of your articles on equivalence testing, and I do not know how to interpret and effect and its corresponding CI, but I do not how to...

12 October 2020 9,046 5 View

Do you believe SARS-Cov-2 vaccines will yield a robust immune response and memory?

In view of the potential for Covid-19 reinfection and the weak immune response and short duration in patients previously infected with Covid-19, many doubts have emerged if the virus is capable of...

03 October 2020 5,689 7 View

No title

I'm currently working in a project in which it would be useful to measure: transmural flux, resistence and potencial difference in mucosa biopsies. I've been reading about this method and as far...

23 September 2020 6,976 1 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View