Alejandro Oñate-Ponce

3 Questions 6 Answers 0 Followers

Questions related from Alejandro Oñate-Ponce

How to improve DBH immunostaining in adult zebrafish?

Hello everybody, I tried to mark Dopamine beta hydroylase (DBH) in adult zebrafish, but I had no good results. I marked TH and DBH and it was just expressed TH in the tissue (postfixed with PFA...

04 October 2019 2,603 3 View

Retina histology using a fixed zebrafish?

Hello everyone, I have a pair of fixed zebrafish's heads (PFA 4%) and I need to know if there'd be any problem using them to study the dopaminergic neurons of the retina. I read a lot about the...

01 July 2019 9,345 2 View

Hi, does anybody have a good (without cracks in brain sections) Nissl staining protocol in Zebrafish or any other fish?

Hello everyone, I have stained brain sections of Zebrafish using Nissl staining and I watched cracks in the tissue :B . It's quite disturbing. Any help will be appreciated. Thanks :)

22 May 2018 1,999 8 View