Minor lab has recently published this article covering practical aspects or crystallographic model refinement (Article Refining the macromolecular model – achieving the best agree...
). This tutorial review offers guidelines for choosing the best settings for the reciprocal-space refinement of macromolecular models and provides practical tips for manual model correction. It also gives practical tips for manual model correction in Coot, modelling of side-chains with poor or missing density, and ligand identification, fitting, and refinement. While we hope that this set of the guidelines will help aspiring protein crystallographers, we also acknowledge that this set is by no means complete or fully applicable to all cases.Our own rules, guidelines, and tips have changed over time due to increased experience and the perpetual evolution of crystallographic software. Moreover, we understand that some crystallographers might disagree with certain suggestions presented in the article. We are always open to discussion on best practices, and I would love to hear feedback, comments, and further suggestions.
If you don’t have an institutional access, please use this link https://www.tandfonline.com/eprint/3UyYh3iNGfhTSJGXyz9K/full (it has a limited number of free copies).
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