Hello
I have a rat model which requires daily s/c injections during pregnancy (Day 1 to Day 18). However, I am struggling to get the rats pregnant despite timing mating during estrus. Even for the control group which receives a daily s/c injection of normal saline, i have had no successful pregnancies.
I wonder whether it is the stress caused to the animal of daily injections and wondered whether others had a similar experience.
Thank you!