How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Pseudomonas that lose their fluorescence?

I am working on Pseudomonas fluorescent strain identification, I found that some of isolate that I have been conserve at 80c last year have loosing their fluorscent appearance on new culture....

09 November 2020 9,181 4 View

Can anyone tell me the list of antibiotics which are sensetive and resistanc to Pseudomonas flourences and Pseudomonas puituda bacteria ?

I am now working on conjugation process between Pseudomonas flourences and Pseudomonas puituda bacteria, so in order to confirm that the genes are transferred, we need to use antibiotics, so...

31 October 2020 509 3 View

How many ways do bacteria have to communicate?

Hello, I am researching into how bacteria communicate. What I've found is that they use quorum-sensing*, and electrical signals**. I've also come across the ability to "speak and listen"***, but I...

03 September 2020 9,168 8 View

The medium used in LasA staphylolytic assay and LasB Elastin congo red assay?

I'm currently collecting references about staphylolytic assay to detect LasA protease activity and elastin congo red assay to detect LasB elastase activity in Pseudomonas aeruginosa, but from the...

03 August 2020 1,160 3 View

Bacterial siderophore production test/modified CAS assay results interpretation?

I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...

26 July 2020 7,194 4 View

NirS qPCR assay underestimating by 2 log?

My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...

20 July 2020 8,070 1 View

Why are bacteria washed with NaCl solutions or some specific buffer before any photolysis experiment?

People who work with bacteria, I have been looking at literature and every time a photolysis experiment to thermally kill bacteria is done, the bacteria are spun down, the supernatant growth...

14 May 2020 9,071 5 View