18 Questions 4 Answers 0 Followers
Questions related from Arnav Padhi
How do I prepare different concentrations of graphene oxide (1, 5, 10ug/ml) for MIC from the powered form of synthesized GO? Please explain the steps.
18 March 2024 5,005 1 View
1. How do we dilute the nanoparticles for doing MIC? 2. How do we determine the final concentration of a nanoparticle after green synthesis?
21 February 2024 2,873 3 View
I am working in the field of Medical Microbiology, My sample size is 300. The biggest problem with such a high number of microbial cultures is contamination and difficulty in maintaining them for...
20 January 2024 1,574 5 View
How the difference in plaque morphology of a bacteriophage affects the adsorption time and latency period to its indicator host?
15 July 2023 3,517 1 View
The pseudomonas aeruginosa isolates in my mac plates are very dry. Can u plz suggest me a method to make them grow again normally. Will taking more culture from the old plate(dry) and streaking it...
19 June 2023 9,270 2 View
When i set up my culture from a plate and do a spot test to check the presence of phages, the culture is not able to grow in the plate and I am unale to see the spots. The culture reaches log...
06 June 2023 9,733 2 View
Why do we do MIC, can disc diffusion assays be enough to check the antibiotic resistance pattern of a bacteria?
24 May 2023 7,135 2 View
Generally peudomonas aeruginosa appear colourless in mac media since it is a non lactose fermenter, but after around a week I m observing that the colour of my pseudomonas culture in mac media has...
22 May 2023 9,253 2 View
I am planning to prepare Urea solution. The stock is in powder form. However while preparing it will be in liquid form. At what temperature it is ideal to store Urea solution?
12 April 2023 3,044 0 View
I am working on pseudomonas. Recently while streaking my isolates on Mac conkey media the isolates are showing red colour instead of pink which is the colour of pseudomonas bacteria. What can be...
05 April 2023 9,720 3 View
I am working on pseudomonas aeruginosa. My bacteria is susceptible to antibiotics, However will it be able to form biofilms?
30 March 2023 8,417 3 View
I am having bacterial contamination in my phages. Even after filtering my phage at 0.22um after a few months, there is a pellet formation occurring below. What should I do to remove contamination...
17 October 2022 6,737 6 View
trations; 1M, 2M, 3M, 4M, 5M. Can someone please tell the protocol to prepare Urea solution?
08 October 2022 2,670 3 View
I had isolated phages against bacillus by treating the purified phage with sm buffer and after two hours of incubation, I transferred the soup and used that as phage lysate after centrifugation,...
01 May 2022 2,679 0 View
How can I prepare a serial dilution of 1024ug/ml antibiotic for MIC. How much volume of the antibiotic will I take so that the final conc of antibiotic becomes 1ug/ml
11 October 2021 1,305 4 View
what can be the fault?
06 February 2019 6,217 4 View
I am working on biofilms for clinical isolates of pseudomonas. What is the best media for growing overnight cultures of pseudomonas as we as biofilms? 2. How do we prepare M63 minimal media...
01 January 1970 2,790 2 View
1. In some of the papers of Pseudomonas isolates it is seen that bacteria that are susceptible to antibiotics are forming strong biofilms. What could be the reason? 2. Bacteria that are MDR show...
01 January 1970 452 0 View
