Hi everyone,
I have a question about How we can predict miRNA binding sites on lncRNAs. Some papers recommend generally some websites such as miRanda, TargetScan or PicTar, but I think none of them are not specified for lncRNAs, I have recenty read a paper named spongeScan: A web for detecting microRNA binding elements in lncRNA sequences, I found this web maybe the useful one that I am looking for. but I have some problem with using it; when I try to load my desired lncRNA FASTA file in corresponding place I always get this message'' The FASTA file you have uploaded does not have the proper extension, please check it" but I tried in many different ways and still I get this message, Has somebody worked with this web or has any experience with this? what is the problem? and also How can I use " Get from Ensemble" option? While I select this option only I have to see " Homo_sapiences GRCH 38.ncRNA, but I want to put or select my desired lncRNA not all. Could you please guide me how I can use properly this web or are there another suitable and informative webs regarding this matter?
Thanks in advance.