What could cause non-specific backgrounds (probes) & DAPI staning in FISH?

In the procedures of FISH, are non-specific backgrounds (probes) and abnormal DAPI staning caused by : - Heat Pretreatment ; - Enzyme digestion ; or - Other? * Are these attached images represent...

04 May 2019 2,769 4 View

Storage of purified PCR product for sequencing ?

01 February 2019 296 6 View

Concentration of RNA for gene expression ?

I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?

31 December 2018 8,434 5 View

Illumina sequencing data analysis and storage ?

In our institute, we are going to establish a Genomic platform (Illumina). For data analysis and storage, what are the minimum requirements in terms of "computer setting (HPC)" using sequencing...

01 February 2018 8,464 5 View

Real-time PCR instruments ?

We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...

01 February 2018 3,532 5 View

Conservation of blood samples for genetic analysis ?

02 March 2016 3,570 4 View

What are the minimum criteria for BSL-3 laboratory ?

31 December 2015 8,472 7 View

Which methods used to studying signal transduction pathways ?

02 March 2015 5,978 5 View

Is the use of a biological cabinet sufficient in a cell culture room (SP1)?

We are going to set up a new cell culture room. What are the necessary procedures to avoid contamination?

11 December 2014 4,310 6 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View