10 Questions 15 Answers 0 Followers
Questions related from Mohamed Lahmadi
In the procedures of FISH, are non-specific backgrounds (probes) and abnormal DAPI staning caused by : - Heat Pretreatment ; - Enzyme digestion ; or - Other? * Are these attached images represent...
05 May 2019 2,847 4 View
Is there any problem if I storage PCR products after purification (from gel or PCR reaction) so that would be used for sequencing (sanger) for less than one month at 4° or -20°C ?
02 February 2019 355 6 View
I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?
01 January 2019 8,498 5 View
In our institute, we are going to establish a Genomic platform (Illumina). For data analysis and storage, what are the minimum requirements in terms of "computer setting (HPC)" using sequencing...
02 February 2018 8,523 5 View
We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...
02 February 2018 3,610 5 View
I need a detailed protocol to conserve blood samples for long period (6-8 months) for extraction of DNA with high quality ?
03 March 2016 3,623 4 View
If someone could aid me about the design and conception and equipments of small laboratory of BSL-3 and the air pressure (-/+) applied in SAS and Rooms.
01 January 2016 8,527 7 View
Which methods are used to studying specific metabolism or signal transduction pathway at multilevels ?
03 March 2015 6,056 5 View
We are going to set up a new cell culture room. What are the necessary procedures to avoid contamination?
12 December 2014 4,373 6 View
- Shord protocol to exctract DNA from FFPE samples
11 November 2014 8,565 0 View
