If someone could aid me about the design and conception and equipments of small laboratory of BSL-3 and the air pressure (-/+) applied in SAS and Rooms.
Hi Mohamed,
what kind of work will be done Inside ? if Risk group 3, the air pressure must be negative on all the air flux, the evacuated air must be filtred throught HEPA filter, So a mecanical room must be designed for Filter decontamination and certification annually. several method for Filter decontamination are used.
what do you mean by Equipment ? depending on what are you working with.
A BSC must be at least type II or above. a double door autoclave, centrifuge with sealed cups or rotors. automatic pippettors....etc
If you have specific question I will be glad to answer.
Hi Mohamed,
what kind of work will be done Inside ? if Risk group 3, the air pressure must be negative on all the air flux, the evacuated air must be filtred throught HEPA filter, So a mecanical room must be designed for Filter decontamination and certification annually. several method for Filter decontamination are used.
what do you mean by Equipment ? depending on what are you working with.
A BSC must be at least type II or above. a double door autoclave, centrifuge with sealed cups or rotors. automatic pippettors....etc
If you have specific question I will be glad to answer.
Thank you very much Mr. Hafid for your responce,
For the air pressure; if we have 2 SAS and Room, it is correct that:
1st SAS positive ---> 2nd SAS negative (Shower, sink, PPE) ----> Room negative
Dear Mohamed,
According to the Canadian public health agency, NIH and CDC, the BSL-3 area must be physically separated from the adjacent laboratories to protect personals outside level 3.
A BSL3 lab shall have a physical separation from the access corridors by way of a vestibule (sas or anteroom) or airlock with self closing doors. Between your Sas 1 and Sas 2 you must have a negative pressure (at least -12.5 Pa) and preferably with interlocked doors. the same for your Sas1 and outside. Never open two doors at once.
It should be a one directional airflow. The deepest negative pressure would be maintained in the zone (main lab) that is considered the highest level of contamination risk
Negative airflow into the laboratory shall the method for containment of airborne BSL3 agents within the lab. The exhaust air shall not be recirculated.
If your Sas-1 has a positive pressure, in case of spilling and problem in HVAC system there is risk of backflow and contamination of the outside area.
Thank you Mr. Hafid ...
This information and explanations are very important.
I am developing BLS1 BSL2 and BSL3 labs.
Here BSL3 is given
Lab 23 Bacterial isolation BSL3 level
Lab 25 Viral isolation BSL3 level.
Other labs are separate preparation labs for virus and bacteria.
And one common DNA RNA extraction lab.
My question is.
What should be pressure in each lab..
How much is the difference in pressure in Pascal
It all depends on what pathogen, what concentration, route of transmission. However following are bare minimum requirements of a BSL3.
1. Air tight construction to withstand 350Pa both positive and negative with suitable materials sealable for gaseous decontamination, seamless, antibacterial, anti fungal and chemical resistant
2. HVAC system with no recirculation, once through, negative pressure cascade wrt to atmosphere, -12.5Pa DP cascade from containment boundary onwards room to room and can go as high as -150Pa depending on number of cascading rooms, exhaust air HEPA filtered preferably through Bag In Bag Out, Supply air through HEPA if risk assessment demands, no possible positive pressure in any failure cases
3. Penetration sealing of all services, envelope for air tightness with special accessories, IP 65 light fittings, switch sockets
4. Air tight doors
5. Wet shower if risk assessment demands (exit through automated shower bath)
6.Drinages inspect able, waste water decontamination with heat sterilization/chemical treatment
7.Biosafety, Biosecurity norms in place, discipline over all
8. Trained and authorized personnel entry
9. Availability of Primary containment systems like BSC/centrifuges etc
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