What could cause non-specific backgrounds (probes) & DAPI staning in FISH?

In the procedures of FISH, are non-specific backgrounds (probes) and abnormal DAPI staning caused by : - Heat Pretreatment ; - Enzyme digestion ; or - Other? * Are these attached images represent...

04 May 2019 2,769 4 View

Storage of purified PCR product for sequencing ?

01 February 2019 296 6 View

Concentration of RNA for gene expression ?

I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?

31 December 2018 8,434 5 View

Illumina sequencing data analysis and storage ?

In our institute, we are going to establish a Genomic platform (Illumina). For data analysis and storage, what are the minimum requirements in terms of "computer setting (HPC)" using sequencing...

01 February 2018 8,464 5 View

Real-time PCR instruments ?

We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...

01 February 2018 3,532 5 View

Conservation of blood samples for genetic analysis ?

02 March 2016 3,570 4 View

What are the minimum criteria for BSL-3 laboratory ?

31 December 2015 8,472 7 View

Is the use of a biological cabinet sufficient in a cell culture room (SP1)?

We are going to set up a new cell culture room. What are the necessary procedures to avoid contamination?

11 December 2014 4,310 6 View

Protocol to isolate manually DNA from FFPE samples ?

- Shord protocol to exctract DNA from FFPE samples

10 November 2014 8,512 0 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View