What could cause non-specific backgrounds (probes) & DAPI staning in FISH?

In the procedures of FISH, are non-specific backgrounds (probes) and abnormal DAPI staning caused by : - Heat Pretreatment ; - Enzyme digestion ; or - Other? * Are these attached images represent...

04 May 2019 2,769 4 View

Storage of purified PCR product for sequencing ?

01 February 2019 296 6 View

Concentration of RNA for gene expression ?

I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?

31 December 2018 8,434 5 View

Illumina sequencing data analysis and storage ?

In our institute, we are going to establish a Genomic platform (Illumina). For data analysis and storage, what are the minimum requirements in terms of "computer setting (HPC)" using sequencing...

01 February 2018 8,464 5 View

Real-time PCR instruments ?

We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...

01 February 2018 3,532 5 View

What are the minimum criteria for BSL-3 laboratory ?

31 December 2015 8,472 7 View

Which methods used to studying signal transduction pathways ?

02 March 2015 5,978 5 View

Is the use of a biological cabinet sufficient in a cell culture room (SP1)?

We are going to set up a new cell culture room. What are the necessary procedures to avoid contamination?

11 December 2014 4,310 6 View

Protocol to isolate manually DNA from FFPE samples ?

- Shord protocol to exctract DNA from FFPE samples

10 November 2014 8,512 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View