7 Questions 8 Answers 0 Followers
Questions related from Lena Gauthier
Dear all, I am looking for a protocol for neuraminidase (NA) treatment of adherent A549 cells (as a control for SNA and MAL-II staining). I am particularly interested in: - Did you fix the...
23 April 2024 9,905 1 View
Dear everyone, I want to label THP-1 cells for IF microscopy but cannot find any detailed protocol on how to label suspension cells without cytospin - is there at all a way of labelling...
14 December 2021 5,284 3 View
I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...
24 October 2018 9,312 6 View
Hi everyone, I know, DNA storage is a big question that everyone answers differently. I would like to know how you would store air-dried DNA pellets. It's genomic DNA and I want to store the DNA...
02 October 2018 7,033 10 View
I try to extract RNA from plant leave material. After extraction by citric acid and isopropanol precipitation, a rather big and sometimes slimy precipitate forms which is not soluble in water....
07 August 2018 6,576 0 View
Hi everyone, I heard that glycerol is better sterile-filtered than autoclaved. Can anybody tell me the appropriate filter size? Thanks in advance!
30 April 2018 5,826 3 View
Until recently I used an ABI 7500 thermo cycler for qPCR and iTaq SYBR Green premix. With this, I always got nice multicomponent plots with a smooth SYBR Green fluorescence signal and a constant...
21 March 2017 3,237 3 View
