Vipul Batra

6 Questions 96 Answers 0 Followers

Questions related from Vipul Batra

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

22 August 2018 3,414 3 View

How can I choose an appropriate Housekeeper if there is a huge intra sample variation in Ct values?

If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...

11 June 2018 4,228 3 View

Why do I get a large number of proteins post PI-PLC treatment of sperm?

I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...

19 March 2018 1,519 1 View

Why do I get Ct value of housekeeping genes sometimes at 18-19 and sometimes at 29-30?

I am looking for gene expression of AMPs in epididymis and testes. However, I ain't been able to replicate the results. There is a lot of variation in Ct values of target as well as Reference...

11 April 2017 5,411 2 View

Is this RNA is degraded or normal ?

I am extracting RNA from epididymis tissue by TRIzol. The nanodrop reading shows 260/280 and 260/230 absolutely fine as 2.0 and 2.01 respectively. However, while running on 1.2% agarose gel I get...

19 December 2016 5,970 18 View

Testing Covid-19

How credible are the test results for COVID-19? What are the False Negative rates of the testing kits used currently? Is it another ticking time bomb?

01 January 1970 7,317 6 View