Vipul Batra

8 Questions 144 Answers 0 Followers

Questions related from Vipul Batra

What is the optimum molecular weight cut-off (MWCO) for concentrating exosomes in PBS?

Hi, I have isolated exosomes from mouse epididymis using SEC in 300ul eluate (PBS) volume. I intend to concentrate the sample in order to increase the protein concentration (for LC-MS/MS) but I am...

30 August 2023 8,980 0 View

What should be the parameter settings for default_input.xml file in X!Tandem for LFQ?

I have QExactive .raw files and using the below mentioned parameters for X!Tandem, however, the pep.xml files appear to have issues and wont open. Why does selecting tandem.params leads to...

03 October 2020 136 0 View

What is the difference between 2(-ddct) and fold change and relative expression?

I have calculated 2 (-ddct) for a set of 6 genes in 5 tissues using GAPDH as reference and one of the tissues as the calibrator. However, after computing the 2 (-ddct) values, which I believe is...

22 August 2018 3,456 3 View

How can I choose an appropriate Housekeeper if there is a huge intra sample variation in Ct values?

If there is a large intra sample variation amongst the housekeeping genes, how can I decide the best housekeeper. The samples are from a abattoir which show large variations in Cq values in the...

11 June 2018 4,241 3 View

Why do I get a large number of proteins post PI-PLC treatment of sperm?

I have treated 100 million spermatozoa with PI-PLC (2U/ml) in sp-TALP medium.The enzyme has specificity for GPI anchor. However, post MS analysis by TPP, approx 300 proteins are identified many...

19 March 2018 1,534 1 View

Why do I get Ct value of housekeeping genes sometimes at 18-19 and sometimes at 29-30?

I am looking for gene expression of AMPs in epididymis and testes. However, I ain't been able to replicate the results. There is a lot of variation in Ct values of target as well as Reference...

11 April 2017 5,424 2 View

Is this RNA is degraded or normal ?

I am extracting RNA from epididymis tissue by TRIzol. The nanodrop reading shows 260/280 and 260/230 absolutely fine as 2.0 and 2.01 respectively. However, while running on 1.2% agarose gel I get...

19 December 2016 5,995 18 View

Testing Covid-19

How credible are the test results for COVID-19? What are the False Negative rates of the testing kits used currently? Is it another ticking time bomb?

01 January 1970 7,331 6 View