I want to quantify the amount of lipid present in lipoprotein by SDS-PAGE analysis. What is the protocol to stain the SDS gel for lipids?
You can use silver staining protocol
http://www.bio-rad.com/LifeScience/pdf/Bulletin_9057.pdf
or Coomassie Blue G-250 Staining
http://www.serva.de/www_root/documents/17524_e_1.pdf
Thank you for your answers.
Apart from this, I want to know about the stain specific for lipids..
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